SILAC media is prepared specifically for labeling experiments involving the use of stable amino acid isotopes. Athena provides several of the most commonly used media without L-arginine, L-lysine, L-leucine and L-methionine, the four mostly commonly used heavy isotope labeled amino acids.
The SILAC ingredients are milled to a fine particle size, blended to give a homogenous mix and filled in moisture proof pouches. Each pouch contains enough medium to prepare 1 liter of medium.
The SILAC medium is prepared as a ready-to-use liquid that is sterile filled and filled in 500 ml bottles. Each bottle is supplied with stock solutions of each of the four missing amino acids. This permits reconstitution of complete medium.